Coding

Part:BBa_K3562000:Design

Designed by: Haoyu Zheng   Group: iGEM20_WHU-China   (2020-10-22)


AiiC (with His-tag)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2071
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2071
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2071
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The TAG will be mutated into a TAA stop codon.


Source

Bacillus sp. 240B1

References